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AIIMS NEW
अखिल भारतीय आयुर्विज्ञान संस्थान, नई दिल्ली
All India Institute Of Medical Sciences, New Delhi

Patient Care

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                     Ph. No.:    011-26549373

Training workshops

Training workshops:  

Conducted Two Day Hands-on Training Workshop on ‘Polymerase Chain Reaction (PCR), RT-PCR & Real-Time PCR Techniques: Application in Diagnostics & Research’, 27th-28th February, 2020.

 

Details of the event-  

The workshop was organized with an objective to introduce students, researchers and clinicians at AIIMS to the basics and Hands-on training for various molecular techniques (DNA, RNA isolation and gel electrophoresis, primer design, PCR, cDNA synthesis and Real-Time PCR). The workshop included-

  • Lecture series on the basics of molecular techniques (principle, procedure, research, clinical applications and troubleshooting).
  • About 150 participant including students, post-docs, faculties and scientists attended the lecture sessions. Hands-on training was imparted to 21 participants and they performed the experiments by themselves.
  • The participants were briefed on data analysis such as melt curve analysis and interpretation.

 

 

Staff

Staff: 

  • Ms. Tanika Giri (Research Assistant)
  • Mr. Amit Sharma (Lab Attendant)

 

EQUIPMENT

  • EQUIPMENT 

 The general facility of the AIIMS CCRF houses multiple molecular biology equipment. Major instruments which has been installed (till now) in General Facility are mentioned below-

 

Details of Major services and equipment provided by General Facility at CCRF (that need prior booking).

SOPs for Main Services

 

Link for downloading Standard operating Procedures (SOPs)

Real Time PCRmachine

(Model: CFX96; Biorad)

    

SOP for Realtime PCR

Polymerase Chain Rection machine

 (Model:SureCycler 8800 Thermal Cycler; Agilent Technologies)

 

 

SOP for PCR Users document

Multimode microplate Reader

(Model:Cytation1; Biotek)

 

 

SOP for Multimode Microplate Reader

Electroporation system

(Model:.Gene Pulser Xcell system; Biorad)

 

 

SOP for Electroporator

Gel Documentation System

(Model: G:Box-Chemi-XX9, Company, Syngene)  

   

SOP for G:Box-Chemi-XX9

Ultra-Centrifuge

(Model:.Optima XPN-100, Beckman Coulter)

  

 

SOP for Ultra-Centrifuge

Refrigerated-Centrifuges

(Model: Heraeus™ Fresco™ 17 Microcentrifuge; Thermo scientific)

  

SOP for Refrigerated Centrifuge

 Multipurpose Centrifuge

Model: Sorvall ST4 Plus Centrifuge, Thermo Scientific 

 SOP for Multipurpose Centrifuge

Homogenizer

(Supplier:JSR)

 

SOP for Homogenizer

Agarose gel electrophoresis set-up

(Supplier:Biorad)

  

SOP for Agarose Gel electrophoresis set-up

ElISA washer and ELlSA reader

(Supplier:Biorad) 

  

SOP for Agarose Gel electrophoresis set-up

 

List of minor equipment (that do not need online booking):  

§  Electronic weighing Balance

§  Ice flaking machine

§  Autoclave

§  pH meter

§  Magnetic stirrers

§  Vortex

§  Water bath

§  Cold microfuge

§  Hot-plate

§  MilliQ and RO water system  

List of new hind equipment (will be available soon):  

§  Digital droplet PCR

(M/s Biorad)

 

Faculty & Scientists

Faculty &  Scientists:   

  • Dr. Kalpana Luthra, Professor of Biochemistry- Coordinator
  • Dr. Ravinder Goswami, Professor of Endocrinology
  • Dr. Parthaprasad Chattopadhyay, Professor of Biochemistry
  • Dr. Lakshmi R, Professor of Cardiac Biochemistry
  • Dr. Deepali Jain, Addl. Professor of Pathology
  • Dr. Archna Singh-1, Associate Professor of Biochemistry
  • Dr. Jayanth Kumar, Associate Professor of Biochemistry  

Scientists:  

  • Dr. Shubbir Ahmed
  • Dr. Vikas Sharma

 

More Articles...

  1. Introduction
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