Overview

Overview:

Proteomics is a high throughput or large-scale comprehensive study of proteome which can be best described as the entire set of proteins that can be expressed by a genome, cell, tissue, or organism at a certain time. The proteomics study can provide information on protein’s differential regulations (for biomarker discovery), variations and modifications, enrichment processes, interacting partners in a network and possible drug target to control a disease.

The Proteomics sub-facility at CCRF, AIIMS provides two different platforms of proteomics experiments –

(1) Discovery/ shotgun/ un-targeted proteomics which is a comprehensive analysis of proteins and

(2) Targeted proteomics using Selected Reaction Monitoring (SRM) or Multiple Reaction Monitoring (MRM) and Parallel Reaction Monitoring (PRM) approaches which are targeted method to validate the protein/s found in the discovery proteomics.

Both approaches are known as bottom-up proteomics approach where digestion of proteins using proteolytic enzymes (like trypsin) are required prior the Mass Spectrometry (MS) analysis. The facility looks to help researchers and clinicians to learn and conduct proteomic experiments that can help to answer research questions associated to human diseases and therapeutics. The cutting-edge Liquid Chromatography couple tandem mass spectrometry (LC-MS/MS) technology platforms will be used which can deliver high-throughput and high-sensitive proteome information.

The facility has four major objectives:

• To provide state-of-the art LC-MS/MS instrumentation facility to facilitate research on qualitative and quantitative aspects of proteins and proteome

• Differential proteome expression and biomarker discovery to flag various human diseases

• Enabling translation of research from Pre-Clinical to Clinical aspects

• Targeted LC-MS/MS based proteomics approach to validate the biomarker in a larger cohort