Immunology Laboratory

Immunology Laboratory

 The immunology section of this department is currently working on

• Immunophenotyping of NK Cells, Dendritic cells, Monocytes/Macrophages, B cells, CD8+ T cells, CD4+ T cells subsets (Th1, Th2, Tregs, Th17)

• Intracellular Cytokine assays (TNF-a, IL-6, IFN-g, IL-2, IL-10, IL-17, etc)

• Serum Cytokine assays (IL-2, IL-4, IL-6, IL-10, TNF, IFN-g, IL-17A)

• Phagocytosis and Respiratory burst assays

Techniques Employed

• PBMC Isolation and Cryopreservation

• Cytometric Bead Array 

• Cellular immunostaining techniques with monoclonal antibodies

• Immune cell culture

• FACS LSR 2 flow cytometer (Becton Dickinson, USA): 6 colour (standardised)

• Western Blotting; ELISA etc.