Selected Publications of the faculty of the department

Publications

Research Publications (last 5 years)

Dr. Y.D. Sharma

1. H. Bora, S. Garg, P. Sen, D. Kumar, P. Kaur, R.H. Khan and Y.D.Sharma. Plasmodium vivax tryptophan-rich antigen PvTRAg33.5 contains alpha helical structure and multidomain architecture. PLoS One, 6, e16294. (2011).

2. V. Lumb, M.K. Das, N. Singh, V. Dev, W. Khan and Y.D. Sharma. Multiple origins of Plasmodium falciparum dihydropteroate synthetase mutant alleles associated with sulfadoxine resistance in India. Antimicrob. Agents Chemother., 55, 2813-7 (2011).

3. V. Lumb and Y.D. Sharma. Novel K540N mutation in Plasmodium falciparum dihydropteroate synthetase confers a lower level of sulfa drug resistance than does a K540E mutation. Antimicrob. Agents Chemother., 55, 2481-82(2011).

4. P. Mittra, N. Singh, and Y.D. Sharma. Plasmodium vivax: immunological properties of tryptophan-rich antigens PvTRAg 35.2 and PvTRAg 80.6. Microbes Infect., 12, 1019-26 (2010).
5. M.K. Das, V. Lumb, P. Mittra, S.S. Singh, A.P. Dash and Y.D. Sharma. High chloroquine treatment failure rates and predominance of mutant genotypes associated with chloroquine and antifolate resistance among falciparum malaria patients from the island of Car Nicobar, India. J. Antimicrob. Chemother., 65, 1258-61 (2010).

6. P. K. Bharti, M.T. Alam, R. Boxer, M.M. Shukla, S.P. Gautam, Y.D. Sharma and N. Singh. Therapeutic efficacy of chloroquine and sequence variation in pfcrt gene among patients with falciparum malaria in central India. Trop. Med. Int. Health, 15, 33-40 (2010).

7. R. Goswami, S. Goel, N. Tomar, N. Gupta, V. Lumb, Y.D. Sharma. Prevalence of clinical remission in patients with sporadic idiopathic hypoparathyroidism. Clin. Endocrinol., 72, 328-33 (2010).

8. N. Tomar, H. Bora, N. Gupta, P. Kaur, Y.D. Sharma, R. Goswami. Presence and significance of a R110W mutation in the DNA binding domain of GCM2 gene in patients with sporadic idiopathic hypoparathyroidism and their family members. Eur. J. Endocrinol., 162, 407-21 (2010).

9. V. Lumb, M.K. Das, N. Singh, V. Dev, W. Khan and Y.D. Sharma. Characteristic of genetic hitchhiking around Plasmodium falciparum dihydrofolate reductase (dhfr) gene associated with pyrimethamine resistance, in India. Antimicrob. Agents Chemother., 53, 5173-80 (2009).

10. H. Bora, M.K. Das, A. Ahmed and Y.D. Sharma. Variations in the mitochondrial DNA markers in the Anopheles (Cellia) sundaicus population from the And aman and Nicobar Island s, India. Acta Trop., 112, 120-4 (2009).

11. P.L. Mehndiratta, P. Bhalla, A. Ahmed and Y.D. Sharma. Molecular typing of methicillin-resistant Staphylococcus aureus strains by PCR-RFLP of SPA gene: a reference laboratory perspective. Indian J. Med. Microbiol., 27, 116-22 (2009).

12. N. Valecha, P. Srivastava, S.S. Mohanty, P. Mittra, S.K. Sharma, P.K. Tyagi, K. Pradhan, Vas Dev, R. Singh, A.P. Dash, Y.D. Sharma. Therapeutic efficacy of artemether-lumefantrine in uncomplicated falciparum malaria in India. Malar. J., 8, 107 (2009).

13. V. Lumb, M. K. Das, P. Mittra, A. Ahmed, M. Kumar, P. Kaur, A. P. Dash, S. S. Singh and Y.D. Sharma. Emergence of an unusual sulfadoxine-pyrimethamine resistance pattern and a novel K540N mutation in dihydropteroate synthetase in Plasmodium falciparum isolates obtained from Car Nicobar Island , India, after the 2004 Tsunami. J. Infect. Dis., 199, 1064-73 (2009).

14. V. Choudhary and Y.D. Sharma. Extensive heterozygosity in flanking microsatellites of Plasmodium falciparum Na(+)/H(+) exchanger (pfnhe-1) gene among Indian isolates. Acta Trop., 109, 241-4 (2009).

15. A. Ahmed and Y.D. Sharma. Ribozyme cleavage of Plasmodium falciparum gyrase A gene transcript affects the parasite growth. Parasitol. Res., 103,751-63 (2008).

16. M.T. Alam, H. Bora, M.K. Das and Y.D. Sharma. The type and mysorensis forms of the Anopheles stephensi (Diptera: Culicidae) in India exhibit identical ribosomal DNA ITS2 and domain-3 sequences. Parasitol. Res., 103, 75-80 (2008).

17. M.T. Alam, H. Bora, P. Mittra, N. Singh and Y.D. Sharma. Cellular immune responses to recombinant Plasmodium vivax tryptophan-rich antigen (PvTRAg) among individuals exposed to vivax malaria. Parasite Immunol., 30, 379-83 (2008).

18. M.T. Alam, H. Bora, N. Singh and Y.D. Sharma. High immunogenecity and erythrocyte-binding activity in the tryptophan-rich domain (TRD) of the 74-kDa Plasmodium vivax alanine-tryptophan-rich antigen (PvATRAg74). Vaccine, 26, 3787-94 (2008).

19. S. Garg, S.S. Chauhan, N. Singh and Y.D. Sharma. Immunological responses to a 39.8kDa Plasmodium vivax tryptophan-rich antigen (PvTRAg39.8) among humans. Microbes. Infect., 10, 1097-105 (2008).

20. R.K. Mehlotra., G. Mattera, M.J. Bockarie, J.D. Maguire, J.K Baird, Y.D. Sharma, M. Alifrangis, G. Dorsey, P.J. Rosenthal, D.J. Fryauff, J.W. Kazura, M. Stoneking and Peter A.Zimmerman. Discordant patterns of genetic variation at two chloroquine-resistant loci in worldwide populations of malaria parasite Plasmodium falciparum. Antimicrob. Agents Chemother., 52, 2212-22 (2008).

21. A. A. Siddiqui, H. Bora, N. Singh, A. P. Dash and Y. D. Sharma. Expression, purification, and characterization of the immunological response to a 40-kilodalton Plasmodium vivax tryptophan-rich antigen. Infect. Immun., 76, 2576-86 (2008).

22. A. Thakur, M. T. Alam, H. Bora, P. Kaur and Y. D. Sharma. Plasmodium vivax: sequence polymorphism and effect of natural selection at apical membrane antigen 1 (PvAMA1) among Indian population. Gene, 419, 35-42 (2008).

23. A. Thakur, M. T. Alam and Y. D. Sharma. Genetic diversity in the C-terminal 42 kDa region of merozoite surface protein-1 of Plasmodium vivax (PvMSP-1(42)) among Indian isolates. Acta Trop., 108, 58-63 (2008).

24. S. Vinayak, M. T. Alam, M. Upadhyay, M. K. Das, V. Dev, N. Singh, A. P. Dash and Y. D. Sharma. Extensive genetic diversity in the Plasmodium falciparum Na+/H+ exchanger 1 transporter protein implicated in quinine resistance. Antimicrob. Agents Chemother. 51, 4508-11 (2007).

25. A. A. Siddiqui, N. Singh, and Y. D. Sharma. Expression and purification of a Plasmodium vivax antigen - PvTARAg55 tryptophan- and alanine-rich antigen and its immunological responses in human subjects. Vaccine, 26, 96-107 (2007).

26. S. Garg, M. T. Alam, M. K. Das, V. Dev, A. Kumar, A. P. Dash, and Y. D. Sharma. Sequence diversity and natural selection at domain I of the apical membrane antigen 1 among Indian Plasmodium falciparum populations. Malar. J., 6, 154 (2007).

27. D. S. Rawat, V. Lumb, Y. D. Sharma, S. T. Pasha and G. Singh. Histone as future drug target for malaria. J. Commun. Dis. 39, 119-28 (2007).

28. S. Vinayak and Y.D. Sharma. Inhibition of Plasmodium falciparum ispH (lytB) gene expression by hammerhead ribozyme. Oligonucleotides 17, 189-200 (2007).

29. M.T. Alam, Richa Agarwal and Y.D. Sharma. Extensive heterozygosity at four microsatellite loci flanking Plasmodium vivax dihydrofolate reductase gene. Mol. Biochem. Parasitol., 153, 178-185 (2007).

30. Asim A. Siddiqui, Rashmi Jalah and Y.D. Sharma. Expression and purification of HtpX-like small heat shock integral membrane protease of an unknown organism related to Methylobacillus flagellatus. J. Biochem. Biophys. Methods., 70, 539-546 (2007).

31. Jeana T DaRe, Rajeev K Mehlotra, Pascal Michon, Ivo Mueller, John Reeder, Y.D. Sharma, Mark Stoneking and Peter A Zimmerman. Microsatellite polymorphism within pfcrt provides evidence of continuing evolution of chloroquine-resistant alleles in Papua New Guinea. Malaria Journal, 6, 34 (2007).

32. M.T. Alam, H. Bora, P.K. Bharti, M.A. Saifi, M.K. Das, Vas Dev, A. Kumar, N. Singh, A.P. Dash, B. Das, Wajihullah and Y.D. Sharma. Similar Trends of Pyrimethamine Resistance Associated Mutations in Plasmodium vivax and P. falciparum. Antimicrobial. Agents Chemother., 51, 857-863 (2007).

33. M.T. Alam, M.K. Das, Vas Dev, M.A. Ansari and Y.D. Sharma. Identification of two cryptic species in the Anopheles (Cellia) annularis complex using ribosomal DNA PCR-RFLP. Parasitol Res., 100, 943-948 (2007).

34. M.T. Alam, M.K. Das, Vas Dev, M.A. Ansari and Y.D. Sharma. PCR-RFLP method for the identification of four members of the Anopheles annularis group of mosquitoes (Diptera: Culicidae). Trans. R. Soc Trop Med. Hyg., 101, 239-244. (2007).

35. Anwar Ahmed, Vanshika Lumb, Manoj K. Das, Vas Dev, Wajihullah and Y.D. Sharma. Prevalence of Mutations Associated with Higher Levels of Sulfadoxine-Pyrimethamine Resistance in Plasmodium falciparum Isolates from Car Nicobar Island and Assam, India. Antimicrobial. Agents Chemother., 50, 3934-3938. (2006).

36. R. Sarin, N. Tomar, D. Ray, Y.D. Sharma and R. Goswami. Absence of pathogenic calcium sensing receptor mutations in sporadic idiopathic hypopararthyroidism. Clin. Endocinol., 65, 359-363. (2006).

37. R. Sarin and Y.D. Sharma. Thioredoxin system in obligate anaerobe Desulfovibrio desulfuricans: Identification and characterization of a novel thioredoxin 2. Gene, 376, 107-115. (2006).

38. P. Mittra, S. Vinayak, H. Chandawat, M.K. Das, N. Singh, S. Biswas, Vas Dev, A. Kumar, M.A. Ansari and Y.D. Sharma. Progressive increase in point mutations associated with chloroquine resistance in Plasmodium falciparum isolates from India. J. Infect. Dis., 193, 1304-1312. (2006).

39. A. Ahmed, M.K. Das, Vas Dev, M.A. Saifi, Wajihullah and Y.D. Sharma. Quadruple mutations in dihydrofolate reductase of Plasmodium falciparum from Car Nicobar Island, India. Antimicrobial. Agents Chemother. 50, 1546-1549. (2006).

40. S. Vinayak, P. Mittra and Y.D. Sharma. Wide variation in microsatellite sequences within each Pfcrt mutant haplotype. Mol Biochem Parasitol. 147, 101-108. (2006).

41. M.T. Alam, M.K. Das, M.A. Ansari and Y.D. Sharma. Molecular identification of Anopheles (Cellia) sundaicus from the Andaman and Nicobar islands of India. Acta. Trop., 97, 10-18. (2006).
 

Dr. Jaya S. Tyagi

Education / Professional Studies:  I.S.C.,Loreto Convent, Lucknow, 1969; B.Sc. from University of Delhi, 1973; M.Sc. from University of Delhi, 1975; Ph.D. from University of Delhi, 1979; Post doctoral training at Laboratory of Molecular Biology, National Cancer Institute, NIH, USA (1979-1983).

Current research interests:

1.           1.     understanding the mechanism and role of key dormancy regulating system, DevR-DevS (also called DosR-DosS), in pathogenesis with special reference to dormancy adaptation of Mycobacterium tuberculosis.

2. developing and validating tools for diagnosis of tuberculosis.
3. exploiting DevR as a novel antitubercular target.
4. dissecting host-M. tb  interactions in vitamin C dormancy model of infection.

Summary of research:

DevR-DevS/DosT signal transduction system of M. tuberculosis.

            In the late 1980s when studies on molecular pathogenesis of M. tb were in their infancy, an innovative RNA subtractive hybridization strategy was designed and successfully performed in my laboratory to identify candidate virulence genes of M. tb. This led to the discovery of a two-component signal transduction system (TCS) designated as DevR-DevS. Subsequently, considerable interest in this regulator was generated on account of its role in mediating the bacterial adaptive response to oxygen limitation, a condition thought to prevail within granulomas where dormant M. tb reside. 

My laboratory has made fundamental contributions towards characterizing and understanding the role of this signaling system  - phosphosignal transfer, promoter mapping, autoregulation, mechanism of transcriptional activation of target genes, characterization of the DevR regulon and cross-talk with the DosT orphan sensor. Furthermore, the level of DevR expression appears to plays a key role in DevR regulon expression, bacterial hypoxic adaptation and virulence in the guinea pig model. A M. tb devR mutant that expresses the N terminal phosphorylation domain (DevR_N) effectively interferes with DevR-mediated signaling. This finding suggests a novel approach to assess TCS-mediated signaling in M. tb without having to generate knock out strains.

DevR is a very attractive and hitherto unexplored target for designing anti-dormancy molecules. Ahomology-based model of DevRwas successfully used for the rational design of inhibitors. A phenylcoumarin derivative inhibited DevR binding to target DNA, down-regulated dormancy genes transcription and drastically reduced survival of hypoxic bacterial cultures. Our findings suggest that A-10 ‘locks’ DevR in an inactive conformation which is unable to bind cognate DNA and induce the dormancy regulon. These results provide proof-of-concept for DevR as a novel target to develop molecules with sterilizing activity against tubercle bacilli.

An in vitro model of dormancy was recently developed wherein tubercle bacilli acquire dormancy phenotype in broth cultures and also within infected THP-1 cells. This model is being exploited to dissect into interactions between host and dormant bacteria and it is hoped that the insights from such analysis will suggest novel targets for interception.

The DevRS system is being further characterized using genetic, biochemical and global molecular approaches to understand its place in the bacterial transcription regulatory network.

Improved laboratory diagnosis of tuberculosis - development and validation of novel approaches and technology.

The rapid, sensitive and specific diagnosis of tuberculosis (TB) is a daunting challenge to microbiology laboratories. To suit the requirements of various laboratories for diagnosing pulmonary and extrapulmonary TB, a multipurpose technology named as USP (universal sample processing) technology was developed. It comprises sample processing, highly sensitive smear microscopy, culture and inhibition-free PCR. The utility of the technology has been validated extensively in a variety of clinical samples. The technology is transferred to Industry. 

Novel approaches of analyzing post-lysis debris for acid-fast bacilli and sample supernatants for M. tb DNA have improved the diagnosis of paucibacillary TB. A recently concluded study confirmed the utility of CSF filtrates in the diagnosis of TB meningitis. The visual detection of amplified DNA by molecular beacons has further simplified the TB PCR test. A disinfection protocol was successfully combined with USP to provide safe handling of infected samples. The detection of M. tb antigens including HspX, Ag85B etc. in CSF specimens has shown great promise for the rapid and efficient diagnosis of tuberculous meningitis. Studies are ongoing to simplify the DNA and antigen-based assays for  TB diagnosis.

Technologies developed:                                

·           Multipurpose TB diagnostic technology (USP technology) encompassing clinical sample processing, smear microscopy, culture and nucleic acid amplification including real time PCR and visual detection. Transferred to Industry

·           High throughput screening assays based on purified DevR and DevS recombinant proteins of M. tuberculosis (M. tb) to identify inhibitors of this signaling system.

·           Cell-based screening assays for compounds active against dormant and replicating mycobacteria.

·           Development of a vitamin C-based dormancy infection model for M. tuberculosis.

Membership of Professional Bodies: Society of Biological Chemists (India), Microbiological Society of India, Indian Society for Cell Biology, American Society of Microbiology.

 Fellowships and membership of professional bodies:

·         National Science Talent Fellowship

·         Member, Guha Research Conference, 1996.

·         Fellow, National Academy of Sciences, India, 1999.

·         Fellow, Indian Academy of Sciences, 2008

·         Fellow,  Indian National Science Academy, 2011

·         JC Bose National Fellow

Awards:  

·         First class First in B.Sc. and M.Sc., University of Delhi

·         First position in Biochemistry in the Agriculture Research Service (ARS) examination , 1978

·         Dr. Kona Sampath Kumar Memorial Prize, University of Delhi, 1985.

·         DBT Short-term Overseas Biotechnology Associateship, 1991. 

·         P.S. Sarma Memorial Award of the Society of Biological Chemists (India), 1999.

·         National Women Bioscientist Award, 1999.

·         New Millennium Science Medal, Indian Science Congress, 2000.

·         Tata Innovation Fellowship, Department of Biotechnology, Govt. of India, 2009

·         Stree Shakti Science Samman 2012

Current positions:  Professor of Biotechnology at A.I.I.M.S.

Honorary Visiting Professor at Centre for Biodesign and Diagnostics, Translational Health Science and Technology Institute, Gurgaon Haryana

Career profile:

1979 - 1983: International Visiting Fellow and Visiting Associate, Ira Pastan’s laboratory, NCI, NIH, USA

1983 - 1987: Scientist C, CSIR Centre for Biochemical Technology (now IGIB), Delhi

1987 - 1991: Associate Professor, AIIMS, New Delhi

1990 - 2001: Additional Professor, New Delhi

Selected Recent Publications (2009-2014):

1. Gautam US, Sikri K, Vashist A, Singh V, Tyagi JS (2014) Essentiality of DevR/DosR interaction with SigA for the dormancy survival program in Mycobacterium tuberculosis. J. Bacteriol. 196:790-799.
2. Sikri K, Tyagi JS (2013) The evolution of Mycobacterium tuberculosis dormancy models. Current Science 105:607-616
3. Haldar S, Sankhyan N, Sharma N, Bansal A, Jain V, Gupta VK, Juneja M, Mishra D, Kapil A, Singh UB, Gulati S, Kalra V, Tyagi JS. (2012) Detection of Mycobacterium tuberculosis GlcB or HspX Antigens or devR DNA Impacts the Rapid Diagnosis of Tuberculous Meningitis in Children. PLoS ONE 7(9): e44630.
4. Majumdar SD, Vashist A, Dhingra S, Gupta R, Singh A, Challu VK, Ramanathan VD, Kumar P, Tyagi JS. (2012) Appropriate DevR (DosR)-Mediated Signaling Determines Transcriptional Response, Hypoxic Viability and Virulence of Mycobacterium tuberculosis PLoS ONE 7(4): e35847.
5. Dhingra S, Kaur K, Taneja NK & Tyagi JS. (2012) DevR (DosR) binding peptide inhibits adaptation of Mycobacterium tuberculosis under hypoxia FEMS Microbiol Lett 330: 66–71
6. Gautam US, Sikri K & Tyagi JS. (2011) Threonine 82 residue of DevR (DosR) is essential for DevR activation and function in Mycobacterium tuberculosis despite its atypical location.  J. Bacteriol. 193:4849-4858
7. Haldar S, Bose M, Chakrabarti P, Daginawala HF, Harinath BC, Kashyap RS, Kulkarni S, Majumdar A, Prasad HK, Rodrigues C, Srivastava R, Taori GM, Varma-Basil M & Tyagi JS. (2011) Improved laboratory diagnosis of tuberculosis : The Indian experience, Tuberculosis (Edinb) 91(5):414-26
8. Chakraborti PK, Matange N, Nandicoori VK, Singh Y, Tyagi JS & Visweswariah SS. (2011) Signalling mechanisms in Mycobacteria Tuberculosis (Edinb). 91(5):432-40
9. Chauhan S, Sharma D, Singh A, Surolia A &Tyagi JS. (2011) Comprehensive insights into Mycobacterium tuberculosis DevR (DosR) regulon activation switch. Nucleic Acids Res. 39(17): 7400-7414
10. Kumar M, Malhotra M, Hanif  M &Tyagi JS. (2011) Exposure of sputum to phenol disinfectant in conjunction with the Universal Sample Processing Solution provides safety to laboratory workers during smear microscopy. J Med Microbiol 60:1410-2
11. Gupta RK, Chauhan S & Tyagi JS. (2011) K182G substitution in DevR or C(8) G mutation in the Dev box impairs protein-DNA interaction and abrogates DevR-mediated gene induction in Mycobacterium tuberculosis. FEBS Journal 278: 2131-2139
12. Tyagi JS. (2011) Value of Sputum microscopy in TB diagnosis and Methods of improving its sensitivity and that of extrapulmonary TB diagnosis. Pg. 164-168 In Proceedings of the Eleventh Sir Dorabji Tata Symposium on Diagnostics in Infections
13. Gautam US, Chauhan S & Tyagi JS. (2011) Determinants outside the DevR C-terminal domain are essential for cooperativity and robust activation of dormancy genes in Mycobacterium tuberculosis. PLoS One. 6(1):e16500.
14. Chauhan S & Tyagi JS (2011) Analysis of transcription at the oriC locus in Mycobacterium tuberculosis Microbiol. Res. 166: 508-514
15. Ray R, Kumar N, Gupta R, Mridha AR, Tyagi JS & Kumar AS (2010) Mesothelial/ monocytic incidental cardiac excrescences (MICE) with tubercular aortitis: report of the first case with brief review of the literature. J Clin Pathol. Jul 29. [Epub ahead of print] doi:10.1136/jcp.2010.080259
16. Taneja NK, Dhingra S, Mittal A, Naresh M & Tyagi JS (2010) Mycobacterium tuberculosis Transcriptional Adaptation, Growth Arrest and Dormancy Phenotype Development Is Triggered by Vitamin C. PLoS One. 5(5):e10860.
17. Majumdar SD, Sharma D, Vashist A, Kaur K, Taneja NK, Chauhan S, Challu VK, Ramanathan VD, Balasangameshwara V, Kumar P & Tyagi JS (2010) Co-expression of DevR and DevRN-Aph proteins is associated with hypoxic adaptation defect and virulence attenuation of Mycobacterium tuberculosis. PLoS ONE 5(2): e9448.
18. Chauhan S, Singh A & Tyagi JS (2010) A single nucleotide mutation in the -10 promoter region inactivates the narK2X promoter in Mycobacterium bovis and Mycobacterium bovis BCG and has application in diagnosis. FEMS Microbiol. Lett. 303:190-196
19. Gupta RK, Thakur TS, Desiraju GR & Tyagi JS (2009) Structure-based design of DevR inhibitor active against non-replicating Mycobacterium tuberculosis J. Med. Chem. 52:6324-6334
20. Chauhan S & Tyagi JS (2009) Powerful Induction of Divergent tgs1-Rv3131 Genes in Mycobacterium tuberculosis Is Mediated by DevR Interaction with a High-Affinity Site and an Adjacent Cryptic Low-Affinity Site. J. Bacteriol. 191: 6075-6081.
21. Chauhan S, Kumar A, Singhal A, Tyagi JS & Prasad HK (2009) CmtR, a cadmium-sensing ArsR–SmtB repressor, cooperatively interacts with multiple operator sites to autorepress its transcription in Mycobacterium tuberculosis. FEBS Journal 276:3428-39.
22. Haldar S, Sharma N, Gupta VK & Tyagi JS (2009) Efficient diagnosis of tuberculous meningitis by detection of Mycobacterium tuberculosis DNA in cerebrospinal fluid filtrates using PCR. J Med Microbiol. 58: 616-624.
23. Malhotra V, Tyagi JS & Clark-Curtiss J.E. (2009) DevR-mediated adaptive response in Mycobacterium tuberculosis H37Ra: Links to asparagine metabolismTuberculosis (Edinb). 89:169-74.

Patents:

Indian:

1. A rapid, efficient, single - tube extraction procedure for the isolation of PCR - amplifiable M.tuberculosis DNA from clinical specimens.  
2. A process for identifying and producing DevR protein of Mycobacterium tuberculosis.
3.  A process for identifying a novel target for the development of therapeutic modalities and drugs effective against tuberculosis.
4.  A simple and fast process for evaluating promoter activity of persistent M. tuberculosis in hypoxic conditions using M. smegmatis as a surrogate host.
5. A method for diagnosis of tuberculosis by smear microscopy, culture and polymerase chain reaction using processed clinical samples and kit thereof.

International:

1.     A process for identifying a novel target for the development of therapeutic modalities and drugs effective against tuberculosis.

2.     A screening method for developing drugs against pathogenic microbes having two-component system.

Dr. H.K. Prasad

1. N.P. Shah, A. Singhal, A. Jain, P. Kumar, S.S. Uppal, M.V.P. Srivatsava and H.K. Prasad. Occurrence of the Overlooked Zoonotic Tuberculosis: Detection of Mycobacterium bovis in Human Cerebrospinal Fluid. J. Clin. Microbiol., 44, 1352-1358. (2006).

2. A. Singhal, A. Jaiswal, V.K. Arora and H.K. Prasad. Modulation of Interferon Gamma Receptor 1 by Mycobacterium tuberculosis: A potential immune evasive mechanism. Infect. Immun., 75, 2500–2510. (2007).

3. P. Kumar, M.V.P. Srivatsava, S Singh and H.K. Prasad. Filtration of CSF improves isolation of Mycobacteria. J. Clin. Microbiol., 46, 2824-2825.

(2008).
4. K. Srivastava, D.S. Chauhan, P. Gupta, H.B. Singh, V.D. Sharma, V.S. Yadav, Shreekumaran, S.S. Thakral, J.S. Dharamdheeran, P. Nigam,

H.K. Prasad and V.M. Katoch. Isolation of Mycobacterium bovis and M. tuberculosis from cattle of some farms in north India – possible relevance in human health. Indian J Med Res., 128, 26-31. (2008).

5. P. Kumar, N.P. Shah, A. Singhal, D.S. Chauhan, V.M. Katoch, S. Mittal, S. Kumar, M.K. Singh, S. Datta Gupta and H.K. Prasad. Association of tuberculous endometritis with infertility and other gynecological complaints of women in India. J. Clin. Microbiol., 46, 4068-4070. (2008).

6. P. Kumar, K. Nath, B. Rath, M.K. Sen, P. Vishalakshi, D.S. Chauhan, V. M. Katoch, S. Singh, S. Tyagi, V. Sreenivas and H.K. Prasad. Visual format for detection of Mycobacterium tuberculosis and Mycobacterium bovis in clinical samples using molecular beacons. J Mol Diagn., 11, 430–438. (2009).

7. S. Chauhan, A. Kumar, A. Singhal, J.S. Tyagi and H.K. Prasad. CmtR, a cadmium-sensing ArsR–SmtB repressor, cooperatively interacts with multiple operator sites to auto-repress its transcription in Mycobacterium tuberculosis. FEBS Journal, 276, 3428–3439. (2009).

8. V. Gupta , A. Jaiswal , D. Behera and H.K. Prasad. Disparity in circulating Peripheral blood dendritic cell subsets and cytokine profile of pulmonary tuberculosis patients compared to healthy family contacts. Hum. Immunol., 71, 682-91. (2010).

9. P. Kumar, M.K. Sen, D.S. Chauhan, V.M. Katoch, S. Singh and H.K. Prasad. Assessment of the N-PCR assay in diagnosis of pleural tuberculosis: Detection of M. tuberculosis in pleural fluid and sputum collected in tand em. PLoS ONE, 5, e10220. (2010).

10. A. Vishnoi, R. Roy, H.K. Prasad and A. Bhattacharya. Anchor-based whole genome phylogeny (ABWGP): a tool for inferring evolutionary relationship among closely related microorganisms. PLoS One, 5:e14159. (2010).
 

Dr. S.N. Das

 1. P. Gaur, M. Mittal, B.K. Mohanti and S.N. Das. Functional genetic variants of TGF-β1 and risk of tobacco-related oral carcinoma in high-risk Asian Indians. Oral Oncol. (in press). (2011).

2. P. Gaur, M. Mittal, B.K. Mohanti and S.N. Das. Functional variants of IL4 and IL6 genes and risk of tobacco-related oral carcinoma in high-risk Asian Indians. Oral Dis. doi: 10.1111/j.1601-0825.2011.01831. (2011).

3. S.N. Das, P. Khare, M.K. Singh, S.C. Sharma. Fas receptor(CD95) and Fas ligand (CD178) expression in patients with tobacco-related intraoral squamous cell carcinoma. Ind J Med Res., 134, 54-60. (2011).

4. A.K. Singh, R. Parshad, S. Pasi, T. Madhavan, S.N. Das, B. Mishra, K. Gill, K. Dalal and S. Dey. Prognostic significance of Cyclooxygenase-2 and response to chemotherapy in invasive ductal breast carcinoma patients by real time surface plasmon resonance analysis. DNA Cell Biol. DOI: 10.1089/dna.2011-1215. (2011).

5. S.N. Das, P. Khare, M.K. Singh and S.C. Sharma. Correlation of Cyclin D1 expression with aggressive DNA pattern in patients with tobacco-related intraoral squamous cell carcinoma. Ind J Med Res., 133, 381-386. (2011).

6. M. Mittal, V. Kapoor, B.K. Mohanti and S.N. Das. Functional variants of COX-2 and risk of tobacco-related oral squamous cell carcinoma in high-risk Asian Indians. Oral Oncol., 46, 622-626. (2010).

7. V. Kapoor, A.K. Singh, S. Dey, S.C. Sharma and S.N. Das. Circulating cyclooxygenase-2 in patients with tobacco-related intraoral squamous cell carcinoma and evaluation of its peptide inhibitors as potential antitumor agent. J Cancer Res Clin Oncol., 136, 1795-1804. (2010).

8. P. Bansal and S.N. Das. Study of antiproliferative activity of Tinospora cordifolia extracts in normal and malignant cells. J Pharmacy Res., 3, 382-385. (2010).

9. R. Gupta, V. Kapuria and S.N. Das. Single nucleotide polymorphisms in TNF-α, TNFR2 gene and TNF-α production in Asian Indians. Immunol Invest., 38, 240-254. (2009).

10. V. Baniasadi and S.N. Das. No evidence for association of PTPN22 R620W functional variant C1858T with type 1 diabetes in Asian Indians. J Cell Mol Med., 12, 1061-1062. (2008).

11. R.K. Somvanshi, R. Subashini, V. Dhanasekaran, G. Arulprakash, S.N. Das and S. Dey. Synthesis, Crystal Structure Cytotoxic and apoptotic activity of 2, 4-Dichloro-6-Methyl Quinoline on human oral carcinoma cell line. J Chem Crystallogr., 38, 381-386. (2008).

12. R. Gupta, S.C. Sharma and S.N. Das. Association of TNF-α and TNFR1 promoters and 3’ UTR region of TNFR2 gene polymorphisms with genetic susceptibility to tobacco related Oral Carcinoma in Asian Indians. Oral Oncol., 44, 445-463. (2008).

13. A. Agarwal, B.K. Mohanti and S.N. Das. Ex vivo triggering of T cell mediated immune responses by autologous tumor cell vaccine in oral cancer patients. Immunopharmacol Immunotoxicol., 29, 95-104. (2007).

14. S.N. Das. Familial ovarian cancer, BRCA1 and BRCA2 mutation, AIIMS Experience. Ind J Med Paed Oncol., 27 (Suppl1), 20. (2007).

15. S.N. Das, V. Baniasadi and V. Kapuria. Association of –308 TNF-α promoter polymorphism with Type-1 Diabetes in North Indians. Int J Immunogenet., 33, 411-416. (2006)

16. S.K. Devarapu, S.C. Sharma and S.N. Das. Triggering of T-cell mediated immune responses with allogenic whole tumor cell vaccine in patients with oral cancer. Immunopharmacol Immunotoxicol., 28, 387-395. (2006).

17. M. Sawhney, M. Mathew, T.M. Valarmathi and S.N. Das. Age related changes in Fas (CD95) and Fas Ligand gene expression and Cytokine profiles in healthy Indians. Asian Pac J Allerg Immunol., 24, 47- 56. (2006)

18. P. Manchanda, S.C. Sharma and S.N. Das. Differential regulation of IL-2 and IL-4 in patients with tobacco related oral squamous cell carcinoma. Oral Dis., 12, 455-462. (2006).

19. V. Baniasadi, N. Narain, R. Goswami and S.N. Das. Promoter region -318 C/T and -1661 A/G CTLA-4 single nucleotide polymorphisms and type 1 diabetes in North Indians. Tissue Antigens., 67, 383-389. (2006).

Dr. Anushree Gupta

107. Anushree Gupta, S. Chandrasekhar, Rahul Pal, Sarita Ahlawat, Om Singh (2001). High expression of human chorionic gonadotropin b-subunit using a synthetic vaccinia virus    promoter. J Mol Endocrinol Jun; 26(3)281-7.
 

108. Anushree Gupta, R Pal, Sarita Ahlawat, Prakash Bhatia, Om Singh (2001). Enhanced immunogenicity of a contraceptive vaccine using diverse synthetic carriers. Vaccine May 14;19(25-26):3384-9.
 

109. Anushree Gupta, S.Chandrasekhar, R Pal, GP Talwar, Om Singh (1998). Identification of novel transmembrane gene sequence and its use in cell-surface targeting of b-subunit of human chorionic gonadotropin. DNA Cell Biol Jul;17(7) :573-81.
 

110. GP Talwar, Om Singh, SK Gupta, SE Hasnain, R Pal, SS Majumdar, S Vrati, A Mukhopadhyay, J Srinivasan, U Deshmukh, S Ganga, A Mandokhot, Anushree Gupta (1997). The HSD-hCG vaccine prevents pregnancy in women: Feasibility study of a reversible safe contraceptive vaccine. Am J Reprod Immunol  Feb;37(2):153-160.
 

111. GP Talwar, Om Singh, R Pal, N Chatterjee, SN Upadhyay, C Kaushic, S Garg, R kaur,M Singh,S.Chandrasekhar, Anushree Gupta (1993) A birth control vaccine is on the horizon for family planning. Ann Med 25:207-212.