रिसर्च धारा वेबसाइट

PUBLICATIONS

Few selected recent publications

1.       Ramakrishnan L, Sachdev HS, Sharma M, Abraham R, Prakash S, Gupta D, Singh Y, Bhaskar S, Sinha S, Chandak GR, Reddy KS, Bhargava S. Relationship of APOA5, PPAR gamma and HL gene variants with serial changes in childhood body mass index and coronary artery disease risk factors in young adulthood. Lipids Health Dis. 2011 May 8;10(1):68

2.   Lakshmy R, Fall CH, Sachdev HS, Osmond C, Prabhakaran D, Biswas SD, Tandon N, Ramji S, Reddy KS, Barker DJ, Bhargava SK. Childhood body mass index and adult pro-inflammatory and pro-thrombotic risk factors: data from the New Delhi birth cohort. Int J Epidemiol. 2010 Jul 26.

3.   Lakshmy R, Ahmad D, Abraham RA, Sharma M, Vemparala K, Das S, Reddy KS, Prabhakaran D. Paraoxonase gene Q192R & L55M polymorphisms in Indians with acute myocardial infarction & association with oxidized low density lipoprotein. Indian J Med Res. 2010 Apr;131:522-9.

4. Ramakrishnan Lakshmy, Ruby Gupta, Dorairaj Prabhakaran, Uma Snehi, K. Srinath Reddy. Utility of Dried Blood Spots for Measurement of Cholesterol and Triglycerides in a Surveillance Study. Journal of Diabetes Science and Technology, March 2010, Volume 4, Issue 2: pages 258-262

5.   Huffman MD, Prabhakaran D, Osmond C, Fall CHD, Tandon N, Lakshmy R, Ramji S,Khalil A, Gera T, Prabhakaran P, Biswas SKD, Reddy KS, Bhargava SK, Sachdev HS, on behalf on the New Delhi Birth Cohort. Incidence of cardiovascular risk factors in an Indian urban cohort. JACC, 2011, 57: 1766-74. 

6.   Jeemon P, Prabhakaran D, Ramakrishnan L, Gupta R, Ahmed F, Thankappan K, Kartha C, Chaturvedi V, Reddy K, Population study Group TS. Association of high sensitive C-reactive protein (hsCRP) with established cardiovascular risk factors in the Indian population. Nutr Metab (Lond) 2011, 8(1): 19

7.   Ramakrishnan L, Thangjam R, Roy A, Singh S, Ramakrishnan L, Seth S, Narang R, Bhargava B. Acute effect effects of tobacco chewing on the systemic, pulmonary and coronary circulation. Am J Cardiovasc Drugs 2011, 11(2) 109-14.

8. Caroline HD Fall, Harshpal Singh Sachdev, Clive Osmond, Ramakrishnan Lakshmy, Sushant Dey Biswas, Dorairaj Prabhakaran, Nikhil Tandon, Siddharth Ramji, K Srinath Reddy, David JP Barker, Santosh K Bhargava.Adult metabolic syndrome and impaired glucose tolerance are associated with different patterns of body mass index gain during infancy; Data from the New Delhi birth cohort. Diabetes Care. 2008 Oct 3.

9. R.Lakshmy, R.Gupta, C.C.Kartha, T.Malathi, P.K.Nigam, N.R.Akarte, U.Sampson, S.Borkotoky, D.N.Doiphode, U.Arora, D.Prabhakaran, K.Srinath Reddy (2008) A simple cost effective quality assurance model for measurement of lipids in a large epidemiological study The Natl. Med J of India 21:279-83.

10. Dorairaj Prabhakaran, Panniyammakal Jeemon,Shifalika Goenka,  Ramakrishnan Lakshmy, K. R. Thankappan, Faruq Ahmed, Prashant P. Joshi, B. V. Murali Mohan, Ramanathan Meera, Mohas S. Das, Ramesh C. Ahuja, Ram Kirti Saran,Vivek Chaturvedi, K. Srinath Reddy, (2009) Impact of a Worksite Intervention Program on Cardiovascular Risk Factors: A Demonstration Project in an Indian Industrial Population Journal of American College of Cardiology  53:1718-28

11. Ramakrishnan Lakshmy and Ruby Gupta. Measurement of Glycated Hemoglobin A1c from Dried Blood by Turbidimetric Immunoassay J Diabetes Science Technology .2009, 3(5): 1203-1206

12. Kumar J, Garg G, Kumar A, Sundaramoorthy E, Sanapala KR, Ghosh S,Karthikeyan G, Ramakrishnan L; Indian Genome Variation Consortium, Sengupta S Single nucleotide polymorphisms in homocysteine metabolism pathway genes: association of CHDH A119C and MTHFR C677T with hyperhomocysteinemia..Circulation Cardiovasc Genet. 2009 Dec;2 (6):599-606.

Introduction

The department of cardiac biochemistry under the Cardiothoracic center (CTC) caters to the inpatients and outpatients of Cardiology and CTVS. In addition to patient care services the department is active in research in the area of developmental origin of adult chronic diseases, telomere biology, endothelial progenitor cells and trans fatty acids.

Patient care

The department under one roof does testing in the areas of clinical chemistry, hematology, coagulation, hormones, cardiac drugs, lipids, catecholamine, marker of heart transplant evaluation, markers of inflammation and in addition assessment of novel risk factors for CAD like homocysteine, lipoprotein(a), apolipoproteins A1 and B. The department has excellent infrastructure and most of the investigations are carried out on automated instruments. Facilities for doing genetic analysis are also available. On an average 250 patients samples are analyzed in the lab every day.

The cardiac biochemistry lab is externally validated through quality assessment scheme (UKNEQAS) accredited to BIRMINGHAM, UK and RIQAS (Randox external quality assessment scheme) and has shown a consistently good performance.

Research

The department is actively involved in research in the following areas:

1)      Understanding the mechanism of developmental origin of adult chronic diseases

2)      Telomere biology

3)      Regulation of endothelial progenitor cells

4)      Single nucleotide polymorphisms in genes involved in lipoprotein metabolism

5)      Non invasive/minimally invasive methods of collection for measurement of analytes

Projects

• Role of Telomere shortening and Telomerase activity on EPC senescence in premature CAD patients and controls
• Evaluation of HDL₂, HDL₃ and apolipoprotein A1 levels in a large Indian industrial population: Association with genetic variants in CETP, LCAT, hepatic lipase and LPL gene

• Relation of APOA-V gene variants to serial changes in childhood body mass index and coronary artery disease risk factors in young adulthood

• LDL particle heterogeneity and its association with other established risk factors for CHD in an Indian industrial population: Role of CETP gene

• Recovery and stability of routine chemistry analytes from filter paper

• Serum fatty acid levels and their correlation with other CVD risk factors in Industrial population from Delhi and Trivandrum