Immunology Laboratory Last Updated On : 04 Sep 2023 Home Introduction Faculty/Staff Faculty Students DMID 3 & 6 year resident Sr. & Jr. Residents Education Lab Services Research Research Project Publication NABL Accreditation NABL Accreditation Immunology Laboratory The immunology section of this department is currently working on Immunophenotyping of NK Cells, Dendritic cells, Monocytes/Macrophages, B cells, CD8+ T cells, CD4+ T cells subsets (Th1, Th2, Tregs, Th17) Intracellular Cytokine assays (TNF-a, IL-6, IFN-g, IL-2, IL-10, IL-17, etc) Serum Cytokine assays (IL-2, IL-4, IL-6, IL-10, TNF, IFN-g, IL-17A) Phagocytosis and Respiratory burst assays Techniques Employed PBMC Isolation and Cryopreservation Cytometric Bead Array Cellular immunostaining techniques with monoclonal antibodies Immune cell culture FACS LSR 2 flow cytometer (Becton Dickinson, USA): 6 colour (standardised) Western Blotting; ELISA etc.