Immunology Laboratory
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Immunology Laboratory
The immunology section of this department is currently working on
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Immunophenotyping of NK Cells, Dendritic cells, Monocytes/Macrophages, B cells, CD8+ T cells, CD4+ T cells subsets (Th1, Th2, Tregs, Th17)
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Intracellular Cytokine assays (TNF-a, IL-6, IFN-g, IL-2, IL-10, IL-17, etc)
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Serum Cytokine assays (IL-2, IL-4, IL-6, IL-10, TNF, IFN-g, IL-17A)
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Phagocytosis and Respiratory burst assays
Techniques Employed
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PBMC Isolation and Cryopreservation
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Cytometric Bead Array
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Cellular immunostaining techniques with monoclonal antibodies
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Immune cell culture
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FACS LSR 2 flow cytometer (Becton Dickinson, USA): 6 colour (standardised)
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Western Blotting; ELISA etc.
