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अखिल भारतीय आयुर्विज्ञान संस्थान, नई दिल्ली
All India Institute Of Medical Sciences, New Delhi

Parasitology Laboratory

Parasitology Laboratory  

Section of Parasitology, Department of Microbiology, is the first dedicated laboratory that provides investigations for diseases caused by parasites. The laboratory has received NABL accreditation. Investigations are available for the diseases caused by various medically important protozoa, helminths and arthropod agents. Many of these diseases are of immense public health importance and include malaria, visceral leishmaniasis, lymphatic filariasis, and intestinal parasitic infections. Microscopic techniques such as direct microscopic examination of unstained preparations of clinical specimens, examination of stained smears and special investigations such as Quantitative Buffy Coat (QBC) assay and immunochromatography test (ICT) are routinely used for some of the diseases. Rapid diagnostic tests are used for diagnosis of different parasitic infections of public health importance and includes antigen detection for malaria (pLDH & HRP-II) and lymphatic filariasis, and antibody detection assays such as, rK 39 test for visceral leishmaniasis, Enzyme Linked Immuno-Sorbent Assay (ELISA) for toxoplasmosis, amoebic liver abscess, cystic hydatid disease, strongyloidiasis, toxocariasis, etc. are being carried out. The section also conducts molecular diagnostic investigations including Multiplex PCR assay for human malaria, and PCR assays for Pneumocystis jiroveci, Microsporidia & free living amoeba.  

S.no.

Test Name

Sample

 

BLOOD (CITRATE) FOR EXAMINATION FOR MICROFILARIA

  • Acridine orange staining for microfilaria (microscopy)

  • Giemsa staining for microfilaria (microscopy)

  • Quantitative buffy coat assay (microscopy)

  • Direct & Concentration mount for microfilaria (microscopy)

  • Filarial Antigen detection test

BLOOD (CITRATE)

 

BLOOD (EDTA) FOR MALARIA WORKUP

  • Acridine orange staining for Malaria parasite (microscopy)

  • Giemsa staining for Malaria parasite (microscopy)

  • Quantitative buffy coat assay for Malaria parasite (microscopy)

  • Malaria parasite antigen detection test

BLOOD (EDTA)

 

MULTIPLEX PCR ASSAY FOR HUMAN MALARIA

BLOOD (EDTA)

 

BLOOD (EDTA) FOR MICROSCOPY FOR PARASITES (OTHER THAN MALARIA)

BLOOD (EDTA)

 

BONE MARROW FOR MICROSCOPIC EXAMINATION FOR LD BODIES (KALA AZAR)

  • Acridine orange staining for LD bodies

  • Giemsa staining for LD bodies

BONE MARROW

(EDTA/SMEAR)

 

BONE MARROW FOR MICROSCOPY FOR PARASITES (OTHER THAN KALA AZAR)

BONE MARROW

(EDTA/SMEAR)

 

BRONCHO-ALVEOLAR LAVAGE FOR MICROSCOPIC EXAMINATION FOR Pneumocystis jiroveci (PCP)

BRONCHO-ALVEOLAR LAVAGE FLUID

 

BRONCHO-ALVEOLAR LAVAGE - PCR FOR Pneumocystis jiroveci (PCP)

BRONCHO-ALVEOLAR LAVAGE FLUID

 

BRONCHO-ALVEOLAR LAVAGE FOR MICROSCOPY FOR PARASITES (OTHER THAN Pneumocystis jiroveci/PCP)

BRONCHO-ALVEOLAR LAVAGE FLUID

 

C.S.F FOR DIRECT MICROSCOPY FOR FREE LIVING AMOEBAE

C.S.F

 

C.S.F FOR MICROSCOPY FOR PARASITES (OTHER THAN FREE LIVING AMOEBAE)

C.S.F

 

DUODENAL ASPIRATE FOR MICROSCOPY FOR PARASITES

DUODENAL ASPIRATE

 

LIVER ABSCESS ASPIRATE FOR MICROSCOPY FOR AMOEBIASIS

LIVER ABSCESS ASPIRATE

 

LIVER ABSCESS ASPIRATE FOR MICROSCOPY FOR PARASITES (OTHER THAN AMOEBIC LIVER ABSCESS)

LIVER ABSCESS ASPIRATE

 

LYMPH NODE ASPIRATE FOR MICROSCOPIC EXAMINATION FOR LD BODIES

  • Acridine orange staining for LD bodies

  • Giemsa staining for LD bodies

LYMPH NODE ASPIRATE

 

LYMPH NODE ASPIRATE FOR MICROSCOPY FOR PARASITES (OTHER THAN LEISHMANIASIS)

LYMPH NODE ASPIRATE

 

PROSTATIC SECRETION FOR MICROSCOPY FOR Trichomonas vaginalis

PROSTATIC SECRETION

 

SERUM FOR ELISA FOR AMOEBIASIS

SERUM

 

SERUM FOR ELISA FOR CYSTIC ECHINOCOCCOSIS

SERUM

 

SERUM FOR ELISA FOR TOXOPLASMOSIS

SERUM

 

SERUM FOR ANTI-Strongyloides IgG ELISA

SERUM

 

SERUM FOR ANTI-Toxocara canis IgG ELISA

SERUM

 

SERUM FOR ANTI-Taenia solium IgG ELISA

SERUM

 

SERUM FOR rK 39 ANTIBODY DETECTION FOR LEISHMANIASIS

SERUM

 

SIGMOIDOSCOPY ASPIRATE FOR MICROSCOPY FOR PARASITES

SIGMOIDOSCOPY ASPIRATE

 

SPLENIC ASPIRATE FOR MICROSCOPY FOR LD BODIES (KALA AZAR)

SPLENIC ASPIRATE

 

SPUTUM FOR MICROSCOPY FOR PARASITES (OTHER THAN Pneumocystis jiroveci/PCP)

SPUTUM

 

STOOL EXAMINATION FOR PARASITES (TROPHOZOITES/CYSTS/OOCYSTS OF COCCIDIA/OVA/LARVAE)

  • Stool direct/concentration mounts microscopy

  • Modified acid-fast staining for coccidian parasites (Cryptosporidium spp./Cyclospora cayetanensis/Cystoisospora belli)

STOOL

 

URETHRAL DISCHARGE FOR MICROSCOPY FOR Trichomonas vaginalis

URETHRAL DISCHARGE

 

URINE FOR MICROSCOPY FOR PARASITES

URINE

 

IDENTIFICATION OF MEDICALLY IMPORTANT ARTHROPODS

ARTHROPOD SPECIMEN

 

IDENTIFICATION OF PARASITIC SEGMENTS/WORMS

SEGMENT/WORM SPECIMEN

 

SPUTUM FOR MICROSCOPIC EXAMINATION FOR Pneumocystis jiroveci (PCP)

  • GMS staining for Pneumocystis jiroveci

SPUTUM

 

SPUTUM FOR PCR FOR Pneumocystis jiroveci (PCP)

SPUTUM

 

STOOL FOR in vitro CULTURE FOR HOOKWORM

STOOL

 

STOOL FOR MICROSCOPIC EXAMINATION FOR MICROSPORIDIA

  • Calcofluor white staining for microsporidia

  • Modified trichrome staining for microsporidia

STOOL

 

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