Bacteriology, Serology & HIC Section

Bacteriology, Serology & HIC Section

Laboratory Services

List of investigations available:

Bacteriology Laboratory (NABL accredited Tests)

S. no	Test performed	Product/ material of the test (Sample)
1	Gram’s stain for Bacteria	Sputum, BAL, mini-BAL, Endotracheal aspirate, Sterile body fluids (pleural, peritoneal, synovial etc), Urine, Tissues, Pus, Swabs, CSF, Bone marrow
2	Culture and susceptibility test: Culture identification (conventional methods and MALDI TOF) and susceptibility testing by disk diffusion method	Blood and bone marrow, Sputum/BAL/miniBAL/Endotracheal aspirate (Respiratory samples), Swab/Pus/ drain fluid/aspirate/catheter tip/biopsy/ urine/PCN/ CSF/sterile body fluids/stool
3	Culture: Automated microbial detection system	Blood, Bone marrow, CSF, Respiratory Sample Sputum, BAL, mini BAL, Endotracheal aspirate), Swab, Pus, Drain fluid, Aspirates, catheter tip, Biopsy, Urine, Percutaneous nephrostomy, Sterile body fluids(Ascitic fluid, Pericardial fluid, Synovial fluid, CSF, Peritoneal fluid, Pleural fluid), Stool.
4	Antimicrobial susceptibility testing by automated system	Blood and bone marrow, Sputum/BAL/miniBAL/Endotracheal aspirate (Respiratory samples), Swab/Pus/ drain fluid/aspirate/catheter tip/biopsy/ urine/PCN/ CSF/Ascitic Fluid, Cerebrospinal Fluid, Pericardial Fluid, Pleural Fluid, Synovial Fluid, Peritoneal fluid /stool
5	Hanging drop for darting motility of Vibrio Cholerae	Stool

 

Other Tests:

S. no	Test performed	Product/ material of the test (Sample)
1	Latex agglutination of CSF for Antigen detection of S. pneumoniae, H. influenzae, N. meningitidis, E. coli, S. agalactiae	CSF
2	Multiplex PCR from CSF for detection of pathogen of Acute Bacterial Meningitis	CSF

 

1. Detection of special resistance mechanisms like MRSA, VRE, ESBL, Colistin Resistance

2. Broth Dilution assay for antimicrobial susceptibility testing

3. Sonication of hardware implants for bacterial culture and sensitivity

4. Typing methods:

   1. Biotyping for species identification

   2. Serotyping for Vibrio cholerae, Salmonella species, Beta haemolytic Streptococci, Shigella species

 

Serology Laboratory: (NABL accredited Tests)

S. no	Test performed	Product/ material of the test (Sample)
1	Anti-Streptolycin O	Serum
2	Venereal disease research laboratory test (VDRL)	Serum
3	Human Treponema Pallidum Antibodies Test (TPHA)	Serum
4	WIDAL	Serum
5	Brucella Antibody Standard Agglutination Test (SAT)	Serum
6	Helicobacter pylori (H.pylori) IgG Antibody detection	Serum
7	Helicobacter pylori (H.pylori) Antigen detection	Stool
8	Serum Procalcitonin Concentration	Serum

Hospital Infection Control Laboratory: This section is involved with the surveillance of health care associated infections in the Institute. The number of health care associated infections including BSI (CLABSI), UTI (CAUTI), SSI, VAP, Hand hygiene compliance is recorded and infection rates determined. Environmental sampling is done to monitor effective decontamination and disinfecting procedures carried out in the hospital.

Central Sterile Supply Department (CSSD) autoclaves are monitored by Biological indicators to check for the functioning of the autoclaves. The sterility of the fluids or pre-sterile articles purchased is tested on the request of the Medical Supply Stores. Monitoring of antimicrobial resistance is done by keeping a record of the resistance pattern of the bacteria causing infections in our hospital. The change in the infection rates and outbreaks are identified and the concerned unit informed in time to take timely control measures. Surveillance of the staff to detect any carrier state in outbreaks is undertaken.

This section also involves with carrying out teaching activities and sensitization of all new health care staff to hospital infection activities.

1. Water Bacteriology: This section tests the bacteriological quality of water in the Institute hospital and residential areas to monitor proper treatment of water being supplied to the Institute

2. External quality assurance Scheme (EQAS)