Name: Dr. Tanu Sagar
Designation: Assistant Professor, Department of Microbiology
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Area of research: Scrub typhus & other Rickettsial diseases, Atypical Bacterial infections (Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila), Lyme’s diseases, Bartonellosis, Leptospirosis.
Name: Dr Priyam Batra
Designation: Assistant Professor
Bacteriology and Serology division
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Areas of research interest:
Antimicrobial resistance
Hospital Infection Control (HIC) and Hospital acquired infections (HAI)
Antimicrobial stewardship
Peritonitis
Bacterial serology
Tuberculosis laboratory
Laboratory Services
The laboratory is NABL accredited, fully equipped to offer complete diagnostic services for diagnosis of tuberculosis (TB), Non-tubercular Mycobacteria (NTM), and drug resistance to various anti-tubercular drugs by molecular as well as conventional methods. The laboratory offers microscopy, culture (both liquid and solid), PCR and Gene-Xpert for diagnosis of TB and NTM. In addition, the conventional drug sensitivity testing in liquid and solid culture media, Gene-Xpert, Line Probe Assay are available for detecting resistance to both first line and second line drugs. The laboratory is equipped with state of art molecular typing techniques for TB. All the techniques are quality assured.
The laboratory carries out research under several funded schemes (Extramural funding support for Whole Genome Sequencing, Early Bactericidal Activity, Therapeutic Drug Monitoring, Point of Care tests (Aptamer based), Novel biomarkers, Rapid molecular drug resistance detection, Molecular Epidemiology of MDR-TB).
List of investigations available:
S. no |
Test performed |
Product/ material of the test (Sample) |
AFB Smear (Ziehl Neelsen Staining) |
Pulmonary & Extrapulmonary samples |
|
Fluorescent staining for M. tuberculosis
|
Pulmonary & Extrapulmonary samples |
|
MGIT 960 Mycobacteria Liquid Cultures. |
Pulmonary & Extrapulmonary samples |
|
Lowenstein Jensen Solid Culture for Mycobacteria |
Pulmonary & Extrapulmonary samples |
|
Drug Susceptibility Test – MGIT 960 Liquid Culture DST first line and second line drugs |
Pulmonary & Extrapulmonary samples |
|
Drug Susceptibility Test – Lowenstein Jensen Solid first line and second line drugs |
Pulmonary & Extrapulmonary samples |
|
Drug Susceptibility Test for NTM |
Pulmonary & Extrapulmonary samples |
|
Gene Xpert MTB/Rif |
Pulmonary & Extrapulmonary samples |
|
Polymerase Chain Reaction targeting Mpt64 gene |
Pulmonary & Extrapulmonary samples |
|
TRUNAT-MTB-RIF detection |
Pulmonary & Extrapulmonary samples |
|
Line Probe Assay (Hain’s) |
Pulmonary & Extrapulmonary samples |
Research (Ongoing)
Whole genome sequencing of M. tuberculosis
Molecular Sequencing & species identification of non-tubercular mycobacteria
Target Sequencing for drug resistance genes of M. tuberculosis
Quantitation and Viability detection using m-RNA Real-time PCR
High resolution melt analysis (HRM analysis) for drug resistance genes of M. tuberculosis
Novel biomarkers for identification of M. tuberculosis
Molecular Epidemiology of MDR-TB
Point of Care tests (Aptamer based)
Proteomics and Metabolomics studies in Pulmonary and Extra-pulmonary TB
Early Bactericidal Activity studies for novel regimen
S.T.D Laboratory
Laboratory Services:
Specimen from the STD clinics and Gynaecology Department are received for screening for the reproductive tract infection (RTI) or the bacterial STDs. The direct smears for Bacterial Vaginosis or vaginitis are screened. Microscopy and cultures for N. gonorrhoeae and the antibiotic resistance monitoring is undertaken. Besides the semen specimen are cultured in case any bacterial infection are suspected. PCR for N. gonorrhoeae and C. trachomatis for uro-genital and extra-genital samples is also available. Culture for T. vaginalis is also available.
List of investigations available:
S.No. |
Test name |
Sample |
1. |
Gram’s stain for GNDC |
Urethral Swab / Discharge |
Endocervical swab / Discharge |
||
Rectal swab |
||
Oro pharyngeal swab |
||
2. |
Gram’s stain for Nugents scoring |
Vaginal Discharge/swab |
3. |
Culture and AST for Neisseria gonorrhoeae
|
Urethral Swab / Discharge |
Endocervical swab / Discharge |
||
Rectal swab |
||
Oro pharyngeal swab |
||
4. |
Routine Bacterial Culture and Susceptibility testing
|
EPS |
Semen |
||
HVS |
||
Vaginal discharge |
||
Endometrial aspirate |
||
5. |
Culture for Trichomonas vaginalis
|
Vaginal discharge /swab |
Urethral Discharge/swab |
||
Urine |
||
6. |
PCR for Neisseria gonorrhoeae |
Urethral Swab / Discharge |
Endocervical swab / Discharge |
||
Rectal swab |
||
Oro pharyngeal swab |
||
7. |
PCR for Chlamydia trachomatis |
Urethral Swab / Discharge |
Endocervical swab / Discharge |
||
Rectal swab |
||
Oro pharyngeal swab |
All India Institute of Medical Sciences
Ansari Nagar, New Delhi - 110029
Board Number : +91-11-26588500 / 26588700
Fax : +91-11-26588663 / 26588641