Confocal Imaging Facility
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Confocal Imaging Facility
The Confocal Imaging Facility in the Department of Physiology is equipped with a Leica TCS SP2 AOBS with an inverted microscope DMIRE2 having 6 visible laser lines (458, 476, 496, 514, 546, 633 nm) and 2 UV laser lines (351, 364 nm). It has 4 PMTs to collect emitted light and 1 PMT to collect transmitted light.
Colocalization of biomolecules using Leica SP2 AOBS
Cytoplasmic expression of Hepatitis E virus fusion protein (A), pORF1EGFP and pORF2EGFP (B) in transfected HepG2 cells. FRET analysis programme is available for further studies.
The Confocal Imaging Facility in the Department of Physiology is equipped with a Leica TCS SP2 AOBS with an inverted microscope DMIRE2 having 6 visible laser lines (458, 476, 496, 514, 546, 633 nm) and 2 UV laser lines (351, 364 nm). It has 4 PMTs to collect emitted light and 1 PMT to collect transmitted light.
Isolated human endometrial epithelial and stromal cells grown on collagen 3-D biomatrix showing evidence of gland formation in vitro. A: Transmitted image; B: Epithelial cell nuclei stained with DAPI; C: Transmitted overlaid image of immunostaining for vascular endothelial growth factor (VEGF); D: Glandular cell immunostaining for VEGF using anti-VEGF Pab & Alexa fluor 633.
Leica SP2 AOBS has z-sectioning ability for 200 mm
Serial Z sectioning of human endometrial gland grown in vitro showing localization of intracellular VEGF immunostaining
Colocalization of biomolecules using Leica SP2 AOBS
Isolated human endometrial epithelial and stromal cells grown on 3-D collagen biomatrix expressing progesterone receptor (B) and insulin like growth factor binding protein-2 (C). Cell nuclei visible from DAPI staining (A), PR and IGFBP-2 colocalized in endometrial cells (D). Immunostaining for PR using anti-PR Mab & Alexa Fluor- 488; for IGFBP2 using anti-IGFBP-2 Pab & Alexa Fluor- 633.
Cytoplasmic distribution of viral proteins tagged with enhanced green fluorescent protein
Cytoplasmic expression of Hepatitis E virus fusion protein (A), pORF1EGFP and pORF2EGFP (B) in transfected HepG2 cells. FRET analysis programme is available for further studies.
Contact Details:- Prof. D.Ghosh, Department of Physiology, AIIMS, New Delhi-29. Room No. 2005. Tel No. 26594625.
